A system for high-resolution respirometry, including fluorometry – enabling simultaneous monitoring of O2 consumption with mt-membrane potential, or ROS, Ca2+, pH, ATP production.

Mitochondria gained an increasing interest over the past years, reflecting a growing awareness of their pivotal role in health and disease. The Oroboros O2k for high-resolution respirometry offers the most advanced technology to analyze mitochondrial function. Combining instrumental accuracy and reliability with versatility in research and biomedical applications, the Oroboros O2k is one of the world-wide leading products on the global market of mitochondrial and cell research. The O2k-FluoRespirometer is Oroboros Instruments flagship for measurement of respiration combined with the control of oxygen levels and monitoring of

• ROS production
• mitochondrial membrane potential
• ATP production
• Ca
• pH

With the O2k as a state-of-the-art laboratory instrument, researchers measure respiration in living cells and mitochondrial preparations from a variety of tissues and blood cells. Multiple titrations can be performed for the addition of substrate combinations, inhibitors and uncouplers to uncover mitochondrial functions. The DatLab software offers a large library of standardized protocols for detailed analysis of mitochondrial pathways, aerobic capacity, and respiratory control. The flexibility of the O2k is based on a unique modular concept. The small-volume chamber module supports respiratory measurements with low numbers of cells and small amounts of biological sample. Fluorometry is combined with respirometric monitoring of oxygen consumption and control of oxygen levels from hyperoxia, normoxia and hypoxia, to anoxic conditions that cannot be obtained in other instruments. The Oroboros O2k is used for bioenergetic and OXPHOS analysis in all types of mitochondrial preparations, including

• isolated mitochondria
• permeabilized tissues
• permeabilized cells
• tissue homogenates
• tissue slices

The Oroboros O2k is dedicated to accuracy and reproducibility, and to resource efficiency with minimum running costs. The O2k combines optimized chamber design, oxygen-tight materials, electrochemical sensors, Peltier-temperature control, and specially developed DatLab software to obtain the unique sensitivity and quantitative resolution of oxygen concentration and oxygen flux, combined with potentiometric and Smart Fluo-Sensors. Oroboros Instruments develops and distributes the gold standard O2k-technology for high-resolution respirometry in mitochondrial and cell research. Oroboros Instruments scientific and technical specialists are in contact with the end-users in Europe, North and South America, and Australasia, and support Oroboros Instruments distributors in Asian countries. Oroboros Instruments provides complete research solutions. These include training in high-resolution respirometry, consultancy in mitochondrial physiology, and international collaborations. Oroboros Instruments science team operates a mitochondrial research laboratory for testing and routine applications of the O2k-technology , and for powering continuous innovations to make a driving force in mitochondrial research. The NextGen-O2k is the new all-in-one MultiSensor instrument, extending the analytical power of high-resolution respirometry. In addition, Oroboros Instruments expand to algal biotechnology and ecology, extending our focus from biomedicine to areas relevant in the Green Deal: environment and climate.